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KMID : 0606620070030040246
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Korean Journal of Fetal Medicine
2007 Volume.3 No. 4 p.246 ~ p.253
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Differential Gene Expression Profiling in Placental Tissue of Severe Preeclamptic and Normal Pregnancies Using cDNA Microarray
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Seo Mi-Won
Kim A-Ri
Kim Ok-Kyoung
Ahn Hyun-Young
Park In-Yang
Lee Young
Kim Sa-Jin
Lee Gui-Se-Ra
Shin Jong-Chul
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Abstract
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Objective: To evaluate the difference of gene expression between placental tissues of severe preeclamptic and
normal pregnancies using cDNA microarray analysis.
Methods: Placental tissues were obtained from 5 cases of normal and severe preeclamptic pregnancies,
respectively. 38,647 genes were screened by oligonucleotide microarrays.
Results: Among 38,647 genes, 146 genes showed up-regulation, while 695 genes showed down-regulation in
severe preeclamptic group. Down syndrome critical region gene 4 (DSCR4), SNF related kinase, and metal-
regulatory transcription factor 1 (MRTF1) were up-regulated, andcatenin (cadherin-associated protein) delta 2,
laminin alpha 3, growth arrest-specific 1 (GAS1), solute carrier family 4 (SCF4), and basic helix-loop-helix domain
containing were significantly down-regulated. According to gene ontologyanalysis, they are associated with signal
transduction, carbohydrate metabolism, cell adhesion, angiogenesis, and angiotensin mediated regulation of renal output.
Conclusion: These results show that cDNA microarray could be used to identify the differentially expressed
genes in severe preeclampsia and could serve as a bioinformative basis in searching novel biomarkers forprediction
and diagnosis of severe preeclampsia.
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KEYWORD
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Severe preeclampsia, Microarray analysis, Gene expression
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